bromocresol purple glucose broth test results

Screening Method to Evaluate Amino Acid

Different culture media have been proposed to be used as screening qualitative procedures the most being formulated as a basal medium that include sources of carbon (glucose) nitrogen (peptone yeast or meat extract) vitamins salt a relative high amount of one (or several) precursor amino acids and a pH indicator (e g bromocresol purple)

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Amino acid metabolism tests

purple The test organism is inoculated into the tubes sealed with mineral oil and incubated Facultatively fermenting bacteria ferment glucose resulting in the production of acid which lower the pH of the medium At this pH bromocresol purple turns yellow from its original purple colour Under anaerobic conditions and low pH the enzyme

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Carbohydrate Metabolism Catalase Oxidase

Examine all tubes for acid and gas production and record your data and results Compare your results with those obtained by other members of the class Glucose Catabolism – The Methyl Red - Voges-Proskauer (MR-VP) Test The Methyl Red-Voges-Proskauer (MR-VP) test is actually two tests that can be performed on a single culture in MR-VP broth

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Lysine Decarboxylase Broth (Taylor)

27 10 05 PS-TV5028Nv03 OXOID ⋅ Am Lippeglacis 4-8 ⋅ D-46483 Wesel PRODUCT INFORMATION Product Name L ysine Decarbox lase Broth (Ta lor) Product Code TV5028N Description With the lysine decarboxylase broth as per Taylor Salmonella spp and some other Enterobacteriaceae can be

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bromocresol purple agar

Bromocresol Purple - an overview | ScienceDirect Topics The method was based on ion-pair formation between cinnarizine and three acidic (sulfonephthalein) dyes namely bromocresol green bromocresol purple and bromocresol blue which induced an instantaneous bathochromic shift of the maximum in the drug spectrum

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Decarboxylation Test: Types uses principles procedure

Decarboxylation Test: Types uses principles procedure and results March 16 2015 Acharya Tankeshwar Bacteriology Biochemical tests in Microbiology Laboratory Diagnosis of Bacterial Disease 7 Decarboxylases are a group of substrate specific enzymes that are capable of reacting with the carboxyl (COOH) portion of amino acids forming alkaline-reacting amines and

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Amino acid metabolism tests

purple The test organism is inoculated into the tubes sealed with mineral oil and incubated Facultatively fermenting bacteria ferment glucose resulting in the production of acid which lower the pH of the medium At this pH bromocresol purple turns yellow from its original purple colour Under anaerobic conditions and low pH the enzyme

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ASMscience

The Moeller's basal media contained peptone beef extract bromocresol purple cresol red pyridoxal and glucose To test decarboxylase activity 1% of the appropriate amino acid was added The medium was poured in narrow tubes as a column of about 2 cm in height and autoclaved after which a layer of about 5 mm sterile paraffin oil was poured

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Glucose Agar Intended Use

Glucose Agar M1589 Ingredients Gms / Litre Tryptone 10 000 Yeast extract 1 500 Dextrose (Glucose) 10 000 Sodium chloride 5 000 Bromocresol purple 0 015 Agar 15 000 Final pH ( at 25C) 7 00 2 **Formula adjusted standardized to suit performance parameters Directions Suspend 41 52 grams in 1000 ml purified / distilled water Heat to boiling to

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Triple Sugar Iron (TSI) Agar Purpose: Determine

Moeller broth is used it contains the specific amino acid glucose and bromocresol purple which is purple at an alkaline pH and yellow at acid pH An organism is capable to decarboxylate the amino acid only after fermenting glucose creating an acid pH after which the decarboxylase enzymes are activated and decarboxylation takes place

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RapidOrnithineDecarboxylase Testfor the Identification of

RAPID ORNITHINE DECARBOXYLASE TEST then 10 g of L-ornithine hydrochloride (Difco) was added The broth was adjusted to pH5 5 with HCl or NaOHandsterilized at 121 C 15 psi for 15 min ThepHwas checked and readjusted to 5 5 if neces- sary The day the tests were performed 1-ml portions of ornithine broth were transferred aseptically to

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Lysine Decarboxylase Broth (Taylor)

27 10 05 PS-TV5028Nv03 OXOID ⋅ Am Lippeglacis 4-8 ⋅ D-46483 Wesel PRODUCT INFORMATION Product Name L ysine Decarbox lase Broth (Ta lor) Product Code TV5028N Description With the lysine decarboxylase broth as per Taylor Salmonella spp and some other Enterobacteriaceae can be

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Methyl Red for MR

Methyl Red (MR) and Voges-Proskauer (VP) broth is used as a part of the IMViC tests as the medium in which both the Methyl Red and Voges-Prosakuer tests can be performed It is a simple broth that contains peptone buffers and dextrose or glucose Different bacteria convert dextrose and glucose to pyruvate using different metabolic pathways

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Glucose Agar Intended Use

Glucose Agar M1589 Ingredients Gms / Litre Tryptone 10 000 Yeast extract 1 500 Dextrose (Glucose) 10 000 Sodium chloride 5 000 Bromocresol purple 0 015 Agar 15 000 Final pH ( at 25C) 7 00 2 **Formula adjusted standardized to suit performance parameters Directions Suspend 41 52 grams in 1000 ml purified / distilled water Heat to boiling to

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Methyl Red for MR

Methyl Red (MR) and Voges-Proskauer (VP) broth is used as a part of the IMViC tests as the medium in which both the Methyl Red and Voges-Prosakuer tests can be performed It is a simple broth that contains peptone buffers and dextrose or glucose Different bacteria convert dextrose and glucose to pyruvate using different metabolic pathways

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Rapid Method Trichophyton rubrum Trichophyton Related

Bromocresol purple-milk solids-glucose medium (BCP-MS-G)wasfirst devised by Fischer and Kane(4) for use in the identification and purification ofdermatophyte isolates The bromocresol purple indicator was added in part to permit the detection of bacterial contaminants in cultures The investigators also discovered however that typical

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Lab 11: Biochemical Tests (Day 2)

Do not discard any test results! You must request the media that you need from the instructor do not take anything without asking UNKNOWN LAB DAY 1: Gram stain your culture and record the results on DATA SHEET and turn in your data sheet to the instructor (you will receive this document back at the beginning of day 2)

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Lab 11: Biochemical Tests (Day 2)

Do not discard any test results! You must request the media that you need from the instructor do not take anything without asking UNKNOWN LAB DAY 1: Gram stain your culture and record the results on DATA SHEET and turn in your data sheet to the instructor (you will receive this document back at the beginning of day 2)

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Identification of cultivable Bifidobacterium species

The carbohydrates fermentation was determined on TPY broth containing bromocresol purple (0 04 g/l) as a pH indicator and supplemented with 1% of the following carbohydrates: lactose sucrose xylose arabinose sorbitol fructose galactose mannose cellobiose raffinose melizitose and melibiose To ensure

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An enrichment broth and differential medium for the

Summary By the use of an enrichment broth and differential media mastitis streptococci and Staph aureus may be isolated from the milk of distant herds Bottles containing glucose lithium chloride potassium tellurite and bromocresol purple are inoculated with the milk samples

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Ornithine decarboxylase Test

The medium used is ornithine decarboxylase broth The medium is a nutrient broth to which 0 5% ornithine is added An important component of the medium is a modest amount of glucose necessary for the process to proceed The p H indicator brom cresol purple is purple at neutral or alkaline/basic p H but turns yellow at p H 5 2 How is the test

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